Products

Superresolution & Confocal Systems

  • Overview
  • MINFLUX
  • MIRAVA POLYSCOPE
  • INFINITY
  • FACILITY
  • STEDYCON
  • Software Overview
  • LiGHTBOX
  • STEDYCON smart control
  • iMSPECTOR

Superresolution & Confocal Modules

  • Overview
  • MINFLUX Module
  • MATRIX Detector
  • TIMEBOW Imaging
  • FLEXPOSURE Illumination
  • RAYSHAPE Mirror
  • TRUESHARP Deconvolution
  • EASY3D
  • RAINBOW Detection
  • STED Lasers
  • Autoalignment
  • Autofocus
  • Excitation Lasers
  • Accessories
  • Custom Solutions

Dyes & Labels

  • Overview
  • abberior STAR
  • abberior LIVE
  • abberior FLUX
  • abberior CAGE
  • abberior Supplies

Shop

  • Dyes by Product Name
  • Dyes by Technique
  • Dyes by Label
  • Mounting Medium
  • Cells and Nanoparticles

Applications

  • Overview
  • Biophysics
  • Cell Biology
  • Live Cell Imaging
  • Material Science
  • Membrane biology
  • Microbiology
  • Neurobiology
  • Physiology
  • Plant Science
  • Virology
  • Zoology

Company

  • People
  • Founders
  • Mission
  • Career
  • Vacancies
  • Locations

News & Events

  • News
  • Events
  • Webinars
  • Past Events

Expertise

  • Knowledge Base
  • FAQ Videos
  • FAQ
  • Publications
  • White Papers
  • Sample Gallery
  • Microscopy Tutorials
  • Webinar Recordings
  • Protocols
  • Support
  • Contact
  • Shop
  • Search
  • EN
    中文
    Language
@abberior.rocks
MENU Contact
MIRAVA POLYSCOPE – All in one and on for all: the perfect image
Science beyond Barriers

abberior instruments

Biophysics, Cell Biology, Physiology

2022
BMC biology

Atheroprone fluid shear stress-regulated ALK1-Endoglin-SMAD signaling originates from early endosomes

Authors:

Paul-Lennard Mendez, Leon Obendorf, Jerome Jatzlau, Wiktor Burdzinski, Maria Reichenbach, Vanasa Nageswaran, Arash Haghikia, Verena Stangl, Christian Hiepen, Petra Knaus

Keywords:

Fluid shear stress; Endothelial cell; Endosome; Endocytosis; Atherosclerosis

Abstract:

Background: Fluid shear stress enhances endothelial SMAD1/5 signaling via the BMP9-bound ALK1 receptor complex supported by the co-receptor Endoglin. While moderate SMAD1/5 activation is required to maintain endothelial quiescence, excessive SMAD1/5 signaling promotes endothelial dysfunction. Increased BMP signaling participates in endothelial-to-mesenchymal transition and inflammation culminating in vascular diseases such as atherosclerosis. While the function of Endoglin has so far been described under picomolar concentrations of BMP9 and short-term shear application, we investigated Endoglin under physiological BMP9 and long-term pathophysiological shear conditions.

Results: We report here that knock-down of Endoglin leads to exacerbated SMAD1/5 phosphorylation and atheroprone gene expression profile in HUVECs sheared for 24 h. Making use of the ligand-trap ALK1-Fc, we furthermore show that this increase is dependent on BMP9/10. Mechanistically, we reveal that long-term exposure of ECs to low laminar shear stress leads to enhanced Endoglin expression and endocytosis of Endoglin in Caveolin-1-positive early endosomes. In these endosomes, we could localize the ALK1-Endoglin complex, labeled BMP9 as well as SMAD1, highlighting Caveolin-1 vesicles as a SMAD signaling compartment in cells exposed to low atheroprone laminar shear stress.

Conclusions: We identified Endoglin to be essential in preventing excessive activation of SMAD1/5 under physiological flow conditions and Caveolin-1-positive early endosomes as a new flow-regulated signaling compartment for BMP9-ALK1-Endoglin signaling axis in atheroprone flow conditions.

< Back to publications
Full article >
linkedin facebook bluesky twitter Instagram

World+49 551 9995 4010USA+1 301 661 0078

© 2025 abberior

Superresolution & Confocal Systems

  • Overview
  • MINFLUX
  • MIRAVA POLYSCOPE
  • INFINITY
  • FACILITY
  • STEDYCON
  • Software Overview
  • LiGHTBOX
  • STEDYCON smart control
  • iMSPECTOR

Superresolution & Confocal Modules

  • Overview
  • MINFLUX Module
  • MATRIX Detector
  • TIMEBOW Imaging
  • FLEXPOSURE Illumination
  • RAYSHAPE Mirror
  • TRUESHARP Deconvolution
  • EASY3D
  • RAINBOW Detection
  • STED Lasers
  • Autoalignment
  • Autofocus
  • Excitation Lasers
  • Accessories
  • Custom Solutions

Dyes & Labels

  • Overview
  • abberior STAR
  • abberior LIVE
  • abberior FLUX
  • abberior CAGE
  • abberior Supplies

Shop

  • Dyes by Product Name
  • Dyes by Technique
  • Dyes by Label
  • Mounting Medium
  • Cells and Nanoparticles

Applications

  • Overview
  • Biophysics
  • Cell Biology
  • Live Cell Imaging
  • Material Science
  • Membrane biology
  • Microbiology
  • Neurobiology
  • Physiology
  • Plant Science
  • Virology
  • Zoology

Company

  • People
  • Founders
  • Mission
  • Career
  • Vacancies
  • Locations

News & Events

  • News
  • Events
  • Webinars
  • Past Events

Expertise

  • Knowledge Base
  • FAQ Videos
  • FAQ
  • Publications
  • White Papers
  • Sample Gallery
  • Microscopy Tutorials
  • Webinar Recordings
  • Protocols
abberior instruments GmbH:
  • Imprint
  • Privacy Policy
  • Terms of Sale
abberior GmbH:
  • Imprint
  • Privacy Policy
  • Terms of Sale
Abberior Instruments America LLC:
  • Privacy Policy
  • Terms of use USA
  • contact
  • manuals
  • service
  • shop