A STED microscope designed for routine biomedical applications

Authors:

Görlitz, F., Hoyer, P., Falk, H., Kastrup, L., Engelhardt, J., & Hell, S. W.

Abstract:

We present a multi-color STED fluorescence microscope providing far-field optical resolution down to 20 nm for biomedical research. The optical design comprises fiber lasers, beam scanners, and a set of active and passive polarizing elements that cooperatively yield an optically robust system for routinely imaging samples at subdiffraction length scales.

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