Progress In Electromagnetics Research
A STED microscope designed for routine biomedical applications
Görlitz, F., Hoyer, P., Falk, H., Kastrup, L., Engelhardt, J., & Hell, S. W.
We present a multi-color STED fluorescence microscope providing far-field optical resolution down to 20 nm for biomedical research. The optical design comprises fiber lasers, beam scanners, and a set of active and passive polarizing elements that cooperatively yield an optically robust system for routinely imaging samples at subdiffraction length scales.