Nanobody conjugated abberior STAR dyes

Indirect immunofluorescence staining is one of the standard approaches for labeling biomolecules of interest, although these antibodies form huge complexes that cause some linkage error between the fluorophore and the biomolecule of interest. Therefore, it is essential to bring the fluorophore as close as possible to the molecule.

Here we go: Because they are ten times smaller – compared to conventional IgG antibodies – our nanobody conjugated abberior STAR dyes offer much better access for superresolution microscopy. This significantly minimizes linkage errors compared to the standard IF staining method.

»During the development of AffiniPureVHH, Jackson ImmunoResearch partnered with abberior to test the STAR dyes on these tiny antibodies. When we tested the conjugates with abberior STAR RED using biological assays, they were remarkably bright compared to several other fluorophores, resulting in very low exposure times and background.«

Katie Wolfe, PhD
R&D Manager at Jackson ImmunoResearch

Polyclonal secondary nanobodies for immunofluorescence labeling

Nanobody anti-
Mouse IgG

Nanobody anti-
Rabbit IgG

Affinipure VHH Secondaries

The combination of abberior STAR dyes conjugated with polyclonal secondary nanobodies (AffiniPure-VHH Secondaries) from Jackson ImmunoResearch offers a new superior solution for IF labeling. While the abberior STAR dyes provide optimal brightness and photostability in STED microscopy, JIR’s polyclonal nanobodies allow binding to multiple binding sites of the primary antibody. This provides high signal amplification and exciting images.

Additional labels are available in our shop.