abberior dyes & labels
Journal of Biomedical Optics
Multifaceted mirror array illuminator for fluorescence excitation-scanning spectral imaging microscopy
Marina Parker, Samuel A. Mayes, Craig M. Browning, Joshua Deal, Samantha Gunn-Mayes, Naga S. Annamdevula, Thomas C. Rich, Silas J. Leavesley
Array illumination, spectral imaging
Hyperspectral imaging (HSI) technologies offer great potential in fluorescence microscopy for multiplexed imaging, autofluorescence removal, and analysis of autofluorescent molecules. However, there are also associated trade-offs when implementing HSI in fluorescence microscopy systems, such as decreased acquisition speed, resolution, or field-of-view due to the need to acquire spectral information in addition to spatial information. The vast majority of HSI fluorescence microscopy systems provide spectral discrimination by filtering or dispersing the fluorescence emission, which may result in loss of emitted fluorescence signal due to optical filters, dispersive optics, or supporting optics, such as slits and collimators. Technologies that scan the fluorescence excitation spectrum may offer an approach to mitigate some of these trade-offs by decreasing the complexity of the emission light path.