Sample gallery
Fluorescence imaging, whether at confocal, STED or MINFLUX resolution, guarantees unique insights into the function and structure of life at the molecular level. Besides the scientific information content, some sample portraits provide simply beautiful images. Enjoy browsing our sample gallery.
the fine art of science
Description
Video of mitochondria in living U2OS cells imaged for 1.5 hours with STED and deconvolved with TRUESHARP image boosting. Mitochondria were visualized with abberior LIVE SiR HaloX staining the outer mitochondrial membrane marker TOMM20. FLEXPOSURE adaptive illumination was used to reduce the light burden on the sample.
Description
A confocal image compared to a MATRIX + TRUESHARP STED image of nuclear pore complexes in mammalian cells stained for NUP96 with abberior STAR RED.
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Description
Living HeLa cells stained with the mitochondrial membrane marker abberior LIVE ORANGE mito, visualizing both outer and inner membranes. Confocal and STED images where deconvolved with TRUESHARP image boosting.
Modules:
Description
Confocal and STED image of a meiotic cell of Chinese spring wheat stained for two synaptonemal complex components.
Sample courtesy: Sepsi Adél, HUN-REN, Centre for Agricultural Research, Martonvásár, Hungary.
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Description
Fixed cells stained for the golgi proteins GM130 (cyan, abberior STAR RED) and giantin (orange, abberior STAR ORANGE), recorded with MATRIX array detection, and deconvolved with TRUESHARP image boosting.
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Description
Two-color 3D MINFLUX movie revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies) coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.
Description
2D MINFLUX nanoscopy of the nuclear pore complex subunits, labeled with abberior FLUX 647 conjugated to JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies). In contrast to confocal microscopy, 2D MINFLUX allows visualization of the shape and arrangement of individual nuclear pore complex subunits. Here, we reach localization precisions of ~ 2 nm in raw localization data.
Description
Two-color 3D MINFLUX revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies) coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.
Description
Paraffin section of gut biopsy stained for Ki67 (abberior STAR ORANGE), Muc2 (abberior STAR RED), and DAPI.
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Description
xz section of a stage 17 Drosophila embryo stained for chitin (abberior LIVE 610, green) and DNA (abberior LIVE 550, cyan).
RAYSHAPE preserves resolution and brightness over the whole sample depth of about 200 µm by dynamically redirecting aberrated light to the right places.
In comparison, mechanical optics using a correction collar can only correct a limited z-range of approximately 20 µm
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Description
Drosophila stage 12 embryo, imaged with RAYSHAPE, stained for tubulin with abberior STAR RED and for DNA with abberior LIVE 550.
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Description
Deep tissue imaging with RAYSHAPE of a stage 17 Drosophila embryo stained for chitin with abberior LIVE 610.
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Description
Clear and detailed 3D rendering of trachea imaged with RAYSHAPE and MATRIX STED. Chitin was stained with abberior LIVE 610.
Modules:
Description
3D STED xz section of a Drosophila embryo trachea, imaged with RAYSHAPE and without abberation correction, depth 15 µm. Chitin was stained with abberior LIVE 610.
Modules:
Description
abberior STAR RED was coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch and used to label tubulin in fixed mammalian cells via indirect immunofluorescence. The STED image was acquired with the STEDYCON system.
Description
Proteins of the nuclear pore complex and the golgi apparatus were stained by indirect immunofluorescence using abberior STAR RED (nuclear pore complex, magenta) and abberior STAR 580 (golgi, green) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.
Description
Vimentin and tubulin were stained by indirect immunofluorescence using abberior STAR RED (vimentin, orange) and abberior STAR 580 (tubulin, cyan) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.
Description
Indirect immunofluorescence staining with abberior STAR dyes coupled to polyclonal anti-mouse secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch shows excellent results in 3-color STED imaging. Staining was performed according to the premix & stain protocol. Vimentin (abberior STAR RED, green), tubulin (abberior STAR 580, magenta) and dsDNA (abberior STAR 460L, blue) were labeled in fixed mammalian cells and visualized with the STEDYCON.
Description
Video of mitochondria in living U2OS cells imaged for 1.5 hours with STED and deconvolved with TRUESHARP image boosting. Mitochondria were visualized with abberior LIVE SiR HaloX staining the outer mitochondrial membrane marker TOMM20. FLEXPOSURE adaptive illumination was used to reduce the light burden on the sample.
Modules:
Description
A confocal image compared to a MATRIX + TRUESHARP STED image of nuclear pore complexes in mammalian cells stained for NUP96 with abberior STAR RED.
Modules:
Description
Living HeLa cells stained with the mitochondrial membrane marker abberior LIVE ORANGE mito, visualizing both outer and inner membranes. Confocal and STED images where deconvolved with TRUESHARP image boosting.
Modules:
Description
Confocal and STED image of a meiotic cell of Chinese spring wheat stained for two synaptonemal complex components.
Sample courtesy: Sepsi Adél, HUN-REN, Centre for Agricultural Research, Martonvásár, Hungary.
Modules:
Description
Fixed cells stained for the golgi proteins GM130 (cyan, abberior STAR RED) and giantin (orange, abberior STAR ORANGE), recorded with MATRIX array detection, and deconvolved with TRUESHARP image boosting.
Modules:
Description
Two-color 3D MINFLUX movie revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies) coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.
Description
2D MINFLUX nanoscopy of the nuclear pore complex subunits, labeled with abberior FLUX 647 conjugated to JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies). In contrast to confocal microscopy, 2D MINFLUX allows visualization of the shape and arrangement of individual nuclear pore complex subunits. Here, we reach localization precisions of ~ 2 nm in raw localization data.
Description
Two-color 3D MINFLUX revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies (secondary nanobodies) coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.
Description
Paraffin section of gut biopsy stained for Ki67 (abberior STAR ORANGE), Muc2 (abberior STAR RED), and DAPI.
Modules:
Description
xz section of a stage 17 Drosophila embryo stained for chitin (abberior LIVE 610, green) and DNA (abberior LIVE 550, cyan).
RAYSHAPE preserves resolution and brightness over the whole sample depth of about 200 µm by dynamically redirecting aberrated light to the right places.
In comparison, mechanical optics using a correction collar can only correct a limited z-range of approximately 20 µm
Modules:
Description
Drosophila stage 12 embryo, imaged with RAYSHAPE, stained for tubulin with abberior STAR RED and for DNA with abberior LIVE 550.
Modules:
Description
Deep tissue imaging with RAYSHAPE of a stage 17 Drosophila embryo stained for chitin with abberior LIVE 610.
Modules:
Description
Clear and detailed 3D rendering of trachea imaged with RAYSHAPE and MATRIX STED. Chitin was stained with abberior LIVE 610.
Modules:
Description
3D STED xz section of a Drosophila embryo trachea, imaged with RAYSHAPE and without abberation correction, depth 15 µm. Chitin was stained with abberior LIVE 610.
Modules:
Description
abberior STAR RED was coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch and used to label tubulin in fixed mammalian cells via indirect immunofluorescence. The STED image was acquired with the STEDYCON system.
Description
Proteins of the nuclear pore complex and the golgi apparatus were stained by indirect immunofluorescence using abberior STAR RED (nuclear pore complex, magenta) and abberior STAR 580 (golgi, green) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.
Description
Vimentin and tubulin were stained by indirect immunofluorescence using abberior STAR RED (vimentin, orange) and abberior STAR 580 (tubulin, cyan) coupled to polyclonal secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch. Images of the fixed mammalian cells were acquired with the STEDYCON.
Description
Indirect immunofluorescence staining with abberior STAR dyes coupled to polyclonal anti-mouse secondary nanobodies (alpaca VHH single domain antibodies) from Jackson ImmunoResearch shows excellent results in 3-color STED imaging. Staining was performed according to the premix & stain protocol. Vimentin (abberior STAR RED, green), tubulin (abberior STAR 580, magenta) and dsDNA (abberior STAR 460L, blue) were labeled in fixed mammalian cells and visualized with the STEDYCON.